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differentiation induction medium  (MedChemExpress)


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    Structured Review

    MedChemExpress differentiation induction medium
    Differentiation Induction Medium, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/differentiation+medium/IBMX/pmc13457214-59-22-34
    Average 97 stars, based on 131 article reviews
    differentiation induction medium - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens.
    Article Snippet: The cells were incubated with taurolithocholic acid (TLCA) (ST4440, Solarbio, Beijing, China) at 0, 25, 50, 100, 150 and 200 μM in serum-free DMEM/F12 for 24 h, and their viabilities were determined by cell counting kit-8 assay (C0037, Beyotime, Shanghai, China). .. The maximum concentration that had no effect on cell viability was chosen as the final concentration of TLCA for the following experiments. p38 MAPK inhibition assay The differentiation medium containing TLCA and p38 MAPK inhibitor SB203580 (152121-47-6, MedChemExpress, Shanghai, China) was added to CPMs after 4 days of differentiation, and the cells were collected after 24 h. ..

    Inhibition:

    Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens.
    Article Snippet: The cells were incubated with taurolithocholic acid (TLCA) (ST4440, Solarbio, Beijing, China) at 0, 25, 50, 100, 150 and 200 μM in serum-free DMEM/F12 for 24 h, and their viabilities were determined by cell counting kit-8 assay (C0037, Beyotime, Shanghai, China). .. The maximum concentration that had no effect on cell viability was chosen as the final concentration of TLCA for the following experiments. p38 MAPK inhibition assay The differentiation medium containing TLCA and p38 MAPK inhibitor SB203580 (152121-47-6, MedChemExpress, Shanghai, China) was added to CPMs after 4 days of differentiation, and the cells were collected after 24 h. ..

    other:

    Article Title: Adipose tissue-secreted Igf2 during promoting adipocyte hyperplasia improves muscle texture caused by high-fat diet through inhibiting myostatin in grass carp (Ctenopharyngodon idellus)
    Article Snippet: With the rapid development of the aquaculture industry, it encounters several challenges, notably the increased costs of fishmeal protein sources.. Although dietary lipid supplementation can decrease protein demand, high-fat diet (HFD) often compromises the aquatic products' muscle texture.. Adipose tissue is an organ for storing energy.

    Transfection:

    Article Title: Comparative analyses of dynamic transcriptome profiles reveal that DUSP1 regulates myogenic differentiation and muscle fiber type transformation
    Article Snippet: .. The differentiation medium containing the p38 MAPK inhibitor SB203580 (152121-47-6, MedChemExpress, Shanghai, China) was replaced 6 h after cell transfection, and the cells were collected 48 h after transfection. ..

    Cell Culture:

    Article Title: Cold exposure-induced β -hydroxybutyrate promotes brown fat mitochondrial lipid droplet contact to ameliorate fatty dysfunction and hepatic steatosis
    Article Snippet: .. For differentiation of 3T3-L1 and SV cells into mature brown adipocytes, cells were cultured to confluence (Day 1), after which the medium was replaced with differentiation medium containing 20 nmol/L insulin (MCE, #HY-P0035), 1 μmol/L rosiglitazone (Sigma, #R2409), 0.5 mmol/L isobutylmethylxanthine (Sigma, #17078), 125 μmol/L indomethacin (Sigma, #17378), 5 μmol/L dexamethasone (Sigma–Aldrich), and 1 nmol/L triiodothyronine (T3, MCE, #HY-A0070AG). ..

    Recombinant:

    Article Title: Asymmetrical paravertebral muscles fibrosis causes progression of adolescent idiopathic scoliosis via myostatin signalling in fibro-adipogenic progenitors
    Article Snippet: Isolated FAPs were cultured in growth medium (GM, high-glucose Dulbecco's modified Eagle's medium [DMEM], #11965092, Gibco] supplemented with 2.5 ng/mL basic fibroblast growth factor [#450-33-10UG, Thermo Fisher Scientific], 20 % fetal bovine serum [FBS, #16000044, Gibco], and 10 % heat-inactivated horse serum [#26050070, Thermo Fisher Scientific]). .. The growth medium was then switched to differentiation medium (DMEM with 10 % FBS, 11.5 mg/mL isobutyl methylxanthine [#HY-12318, MCE], and 1 mg/mL insulin [#HY-P0035, MCE]) supplemented with 1 mM dexamethasone (Dex, #HY-14648, MCE) or 100 nM recombinant myostatin (#HY- P72632 , MCE). ..



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    RNA-seq profiling of human adipose-derived stem cells (hASCs) after 21 days of culture in myogenic <t>differentiation</t> <t>medium.</t> (a) Principal component analysis (PCA) based on transcriptome expression values (FPKM), showing clustering of biological replicates for Monolayer (2D), PCL, and Fibril conditions. (b) Venn diagram showing the overlap of detected genes among Monolayer, PCL, and Fibril groups (numbers indicate gene counts in each intersection). (c) Gene Ontology (GO) Biological Process (BP) over-representation analysis (ORA) for differentially expressed genes in 2D vs nFMBs (left) and PCL-mFiBs vs nFMBs (right); bars are plotted as −log10 (adjusted p value), with terms enriched among genes upregulated in the first condition shown to the right (red) and terms enriched among genes downregulated in nFMBs shown to the left (blue). (d) KEGG pathway enrichment analysis for differentially expressed genes between PCL-mFiBs and nFMBs groups; dot size represents the number of genes mapped to each pathway (Count), dot color indicates adjusted p value, and the x-axis denotes Gene Ratio. (e) Category network plot (CNP; category–gene network plot) for the PCL-mFiBs vs nFMBs comparison, visualizing representative enriched GO BP terms and their associated genes; gene nodes are colored by fold change and term nodes reflect enrichment significance. (f–i) Heatmaps of selected genes associated with representative GO terms: GO:0000280 (nuclear division), GO:0030198 (extracellular matrix organization), GO:0003012 <t>(muscle</t> system process), and GO:0007519 <t>(skeletal</t> muscle tissue development), respectively; expression patterns are shown across 2D, PCL-mFiBs, and nFMBs, with gene symbols listed alongside each heatmap.
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    Image Search Results


    RNA-seq profiling of human adipose-derived stem cells (hASCs) after 21 days of culture in myogenic differentiation medium. (a) Principal component analysis (PCA) based on transcriptome expression values (FPKM), showing clustering of biological replicates for Monolayer (2D), PCL, and Fibril conditions. (b) Venn diagram showing the overlap of detected genes among Monolayer, PCL, and Fibril groups (numbers indicate gene counts in each intersection). (c) Gene Ontology (GO) Biological Process (BP) over-representation analysis (ORA) for differentially expressed genes in 2D vs nFMBs (left) and PCL-mFiBs vs nFMBs (right); bars are plotted as −log10 (adjusted p value), with terms enriched among genes upregulated in the first condition shown to the right (red) and terms enriched among genes downregulated in nFMBs shown to the left (blue). (d) KEGG pathway enrichment analysis for differentially expressed genes between PCL-mFiBs and nFMBs groups; dot size represents the number of genes mapped to each pathway (Count), dot color indicates adjusted p value, and the x-axis denotes Gene Ratio. (e) Category network plot (CNP; category–gene network plot) for the PCL-mFiBs vs nFMBs comparison, visualizing representative enriched GO BP terms and their associated genes; gene nodes are colored by fold change and term nodes reflect enrichment significance. (f–i) Heatmaps of selected genes associated with representative GO terms: GO:0000280 (nuclear division), GO:0030198 (extracellular matrix organization), GO:0003012 (muscle system process), and GO:0007519 (skeletal muscle tissue development), respectively; expression patterns are shown across 2D, PCL-mFiBs, and nFMBs, with gene symbols listed alongside each heatmap.

    Journal: Bioactive Materials

    Article Title: Muscle-fiber-inspired nanofibrillar microbundles induce myogenic differentiation in human adipose-derived stem cells

    doi: 10.1016/j.bioactmat.2026.03.020

    Figure Lengend Snippet: RNA-seq profiling of human adipose-derived stem cells (hASCs) after 21 days of culture in myogenic differentiation medium. (a) Principal component analysis (PCA) based on transcriptome expression values (FPKM), showing clustering of biological replicates for Monolayer (2D), PCL, and Fibril conditions. (b) Venn diagram showing the overlap of detected genes among Monolayer, PCL, and Fibril groups (numbers indicate gene counts in each intersection). (c) Gene Ontology (GO) Biological Process (BP) over-representation analysis (ORA) for differentially expressed genes in 2D vs nFMBs (left) and PCL-mFiBs vs nFMBs (right); bars are plotted as −log10 (adjusted p value), with terms enriched among genes upregulated in the first condition shown to the right (red) and terms enriched among genes downregulated in nFMBs shown to the left (blue). (d) KEGG pathway enrichment analysis for differentially expressed genes between PCL-mFiBs and nFMBs groups; dot size represents the number of genes mapped to each pathway (Count), dot color indicates adjusted p value, and the x-axis denotes Gene Ratio. (e) Category network plot (CNP; category–gene network plot) for the PCL-mFiBs vs nFMBs comparison, visualizing representative enriched GO BP terms and their associated genes; gene nodes are colored by fold change and term nodes reflect enrichment significance. (f–i) Heatmaps of selected genes associated with representative GO terms: GO:0000280 (nuclear division), GO:0030198 (extracellular matrix organization), GO:0003012 (muscle system process), and GO:0007519 (skeletal muscle tissue development), respectively; expression patterns are shown across 2D, PCL-mFiBs, and nFMBs, with gene symbols listed alongside each heatmap.

    Article Snippet: For HSkMCs, myogenic differentiation was induced using Skeletal Muscle Differentiation Medium (ATCC, Manassas, USA).

    Techniques: RNA Sequencing, Derivative Assay, Cell Characterization, Expressing, Comparison